[대학원 생명과학과 세미나 안내]
연사 : 이윤희 교수(연세대학교 약학대학)
연제 : Deconstructing cellular and metabolic heterogeneity of adipose tissue in health and disease
일시 : 2018년 5월 25일 (금) 오후 5시
장소 : 하나과학관 A동 B131호
초청교수 : 구승회 교수
Abstract
Recruitment of brown/beige adipocytes (BA) in white adipose tissue (WAT) involves proliferation and differentiation of adipocyte stem cells (ASC) in concert with close interactions with resident immune cells. To deconvolve stromal cell heterogeneity in a comprehensive and unbiased fashion, we performed single cell RNA sequencing (scRNA-seq) of > 33,000 stromal/vascular cells from epididymal WAT (eWAT) and inguinal WAT (iWAT) under control conditions and during b3-adrenergic receptor (ADRB3) activation. scRNA-seq identified distinct ASC subpopulations in eWAT and iWAT that appeared to be differentially poised to enter the adipogenic pathway. ADRB3 activation triggered the dramatic appearance of proliferating and non-proliferating ASC in eWAT, whose differentiation into BA could be inferred from a single time point. scRNA-seq identified various immune cell types in eWAT, including a proliferating macrophage subpopulation that occupies adipogenic niches. These results demonstrate the power of scRNA-seq to deconstruct adipogenic niches and suggest novel functional interactions among resident stromal cell subpopulations.