[대학원 생명과학과 세미나 안내]
연사 : 김현우 교수(카이스트 생명과학과)
연제 : Irisin, its receptor and the links between exercise and health
일시 : 2022년 04월 01일 (금) 오후 4시 30분
장소 : 대면(207호 화상회의실) 및 온라인 화상 강의로 진행됩니다.
초청교수 : 구승회 교수
Abstract
Exercise has beneficial effects on several organs. These effects are often mediated by myokines, muscle secreted factors for tissue crosstalk. Irisin is a myokine induced by exercise in skeletal muscle. Irisin is a polypeptide of 12kDa that is cleaved from a type I membrane protein called FNDC5. FNDC5 is expressed mainly from skeletal muscle, heart, and brain. FNDC5 mRNA increases in adult human muscles with several forms of endurance exercise. Advanced tandem Mass Spectrometry has demonstrated that human irisin circulates at “hormone-like” levels and increases as a consequence of endurance exercise. Since its discovery in 2012, irisin has been shown to affect bone, fat, and brain. In many cases, irisin’s effects are reminiscent of those derived from physical exercise, including improved cognition and bone remodeling in mice.
Here, we identified the major receptor for irisin as the αV integrin family and its cofactor as cluster of differentiation 81 (CD81) with quantitative proteomics using mass spectrometry. Irisin treatment increased phosphorylation of focal adhesion kinase (FAK), and genetic deletion of CD81 or integrin αV inhibitors blunted the signaling. Irisin treatment enhanced thermogenic fat cell proliferation and genetic deletion of integrin β1 or integrin β5, dimer partners of integrin αV, prevented irisin-induced FAK phosphorylation. Genetic deletion of CD81 blocked irisin-induced thermogenic fat cell proliferation in vitro and worsened metabolic phenotypes upon high-fat diet challenge. Overall, this data suggests irisin treatment as a therapeutic approach to metabolic diseases such as obesity and type 2 Diabetes.