[대학원 생명과학과 세미나 안내]
연사 : 현재경 교수(부산대학교 의과대학 융합의학교실)
연제 : Latest Advances and Prospectives of Cryo-Electron Microscopy
일시 : 2022년 04월 08일 (금) 오후 4시 30분
장소 : 대면(207호 화상회의실) 및 온라인 화상 강의로 진행됩니다.
초청교수 : 우재성 교수
Abstract
Transmission electron microscope (TEM) is an optical instrument that utilizes electron wave as a beam source. Based on Rayleigh criterion, sub-nanometer wavelength of electron radiation allows for imaging at atomic resolution. Since its invention in the early 1930s by Ernst Ruska, TEMs have made enormous contributions in physics, chemistry, material science and life science.
In biological sciences, TEM offers high resolution and versatility that can visualize samples that range from atomic details of molecular structures of proteins to cellular ultrastructures. In particular, technology that can produce the reconstruction of 3D electron coulomb potential maps from the 2D projection images of TEM, so-called 3D electron microscopy (3DEM), has led significant advancements in our understanding of biological processes in recent years. Most pivotal breakthrough in high-resolution 3DEM is the development of cryo-electron microscopy (cryo-EM). Cryo-EM is a technique that involves combination of cryogenic sample preparation, low electron dose imaging and computational image processing. Developments in instrumentations and software algorithms in the past decade have dramatically improved achievable resolution by cryo-EM, which can now resolve individual atoms in frozen-hydrate proteins.
In this presentation, basic principles in cryo-EM and recent technical breakthroughs will be introduced, and current efforts in technical development of electron tomography for in situ biological imaging will be discussed.