[대학원 생명과학과 세미나 안내]
연사 : Nico Dissmeyer 박사(Leibniz Institute of Plant Biochemistry (IPB))
연제 : The N-end rule pathway – from phenotypes to applications
일시 : 2017년 7월 4일 (화) 오후 4시30분
장소 : 하나과학관 A동 101호
초청교수 : 송현규 교수
Abstract
My lab is interested in proteostasis and posttranslational protein modification via the N-end rule pathway of targeted protein degradation as part of the Ubiquitin proteasome system. Our research includes: (1) enzymatic mechanisms and their regulation; (2) substrate identification and biological integration ('phenotypes’) of the N-end rule system; (3) roles under stress conditions versus the normal environment in plant development and fitness using Arabidopsis as the model; and (4) harnessing the N-end rule pathway for genetic and biotechnological applications in various multicellular organisms. We developed assays and artificial substrates highly specific for this pathway to identify and characterize novel enzymatic modifiers and approach potential substrates via differential proteomics and knowledge-based pipelines. We strive for biotechnological application and translation of our work which has allowed to generate "phenotypes on demand" by conditionally accumulating target proteins and by this switch both their function and activity (Faden et al., Nature Comms. 2016). For further information, please check www.dissmeyerlab.org
Keywords: The N-end rule pathway, N-terminal arginylation, protein recognition, proteolysis, arginyl tRNA transferase, ubiquitin E3 ligase, biotechnology, molecular farming