[대학원 생명과학과 세미나 안내]
연사 : 노성훈 박사(Bioscience Division, SLAC National Accelerator Laboratory, Stanford University)
연제 : Studying macromolecular structures using cryoEM
일시 : 2017년 9월 20일 (수) 오후 4시
장소 : 하나과학관 A동 207호
초청교수 : 송현규 교수
Abstract
The vacuolar H+-ATPase (V-ATPase) is an essential proton pump found in every eukaryotic cell. Misregulated or defective V-ATPase leads to widespread human diseases such as renal tubular acidosis, neurodegeneration and cancer, with many disease-causing mutations found in the enzyme’s Vo proton channel sector. Here we report the cryo-electron microscopy structure of lipid nanodisc-reconstituted yeast Vo determined at 3.5 Å resolution. Isotropic map resolution allowed building of a ~95% complete all-atom model of the complex, providing unprecedented detail for the residues involved in proton translocation. Based on the structure and available mutagenesis data, we suggest a mechanism of proton transport across VO. The structure also suggests the mechanism of disease-causing mutations in human VO, thereby illuminating new avenues for therapeutic intervention.