[대학원 생명과학과 세미나 안내]
연사 : 심상희 교수(고려대 화학과)
연제 : Super-resolution fluorescence microscopy for subcellular structures and dynamics
일시 : 2017년 12월 15일 (금) 오후 5시
장소 : 하나과학관 A동 B131호
초청교수 : 송현규 교수
Abstract
Super-resolutionfluorescence microscopy opens new windows for visualizing ultrastructuraldynamics. When applied to living cells, super-resolution fluorescence microscopy suffers from the limited length oftime-lapse series due to high intensities of illumination and photobleaching offluorophores. To overcome the challenges in photobleaching, we use a fluorogen-binding protein, UnaG for overcoming the photobleaching limit by controllingthe switching kinetics and by supplying a large excess of fluorogenes. UnaG fluorescence can be switched off by bluelight and then, recovered by replacing the damaged fluorogen with a fresh onein solution for hundreds of cycles. These switching properties enabledlong-term super-resolution imaging based on single-molecule localization, with significant improvement (about 10-fold) in the number of independentsuper-resolution snapshots. With this tool in hand, we demonstrate long-term (tens of minutes) observation of the endoplasmic reticulumn (ER) and genomic loci using CRISPR/Cas9 system.