[대학원 생명과학과 세미나 안내]
연사 : 신찬석 교수(서울대학교 응용생물화학부)
연제 : MicroRNAs and Disease Resistance Genes in Pepper
일시 : 2018년 3월 9일 (금) 오후 5시
장소 : 하나과학관 A동 B131호
초청교수 : 김옥매 교수
Abstract
MicroRNAs (miRNAs) are a class of non-coding RNAs approximately 21 nt in length which play important roles in regulating gene expression in plants. To investigate the functions of miRNAs in hot pepper (Capsicum annuum), we employed transcriptome approaches including RNA-Seq, small RNA-Seq and Degradome-Seq to comprehensively identify small RNAs and their targets. From this, we identified several highly abundant 22-nt miRNA families that target conserved domains in NB-LRRs and trigger the production of phased secondary siRNAs. In addition, our results suggested that interaction between miR-482 family and NB-LRR disease resistance proteins is likely to serve as a conserved trigger for plant defense mechanism. This work provides an expanded picture of pepper miRNAs in relation to NB-LRR regulation, providing a basis for understanding the functional roles of miRNAs in disease resistance in pepper.