[대학원 생명과학과 세미나 안내]
연사 : 허준호 교수(경희대학교 의과대학 병리학교실)
연제 : CRISPR genome editing and deep sequencing based mechanistic study of diseases
일시 : 2018년 4월 20일 (금) 오후 5시
장소 : 하나과학관 A동 B131호
초청교수 : 지성욱 교수
Abstract
Recent development of high throughput sequencing andCRISPR genome editing technology have brought great advances in the understanding of molecular mechanisms underlying diseases. We demonstrated that CRISPR-Cpf1,a recently found class 2/type V CRISPR RNA-guided endonuclease that is distinct from the common CRISPR-Cas9, can be an efficient method to generate mutant mice for diseases modelling. Similarly, we applied CRISPR for generating severe combined immunodeficiency (SCID) mini-pig models via knock out of Rag2 gene and somatic nuclear transfer. Next, we applied a modified CRISPR base editing system to study glioma linked mutations. Previous studies reported that patient derived glioma cells with Isocitrate dehydrogenase I (IDH1) R132 single base mutation are unculturable. To this end, we used CRISPR base editing to introduce the aforementioned point mutation into a glioma cell line to study the molecular and cellular aspect of the mutation with respect to cancer progression. We next applied CRISPR to study bacterial urinary tract infection, where we generated KO bacterial strains to identify a novel receptor for quorum sensing molecules that regulate bacterial biofilm formation and pathogenicity.