[대학원 생명과학과 세미나 안내]
연사 : 성지혜 박사(KIST 뇌과학연구소)
연제 : Optical sensing and control of protein activity by fluorescent protein
일시 : 2020년 11월 27일 (금) 오후 5시
장소 : 온라인 화상 강의로 진행됩니다.
초청교수 : 오은규 교수
Abstract
Recent advances in fluorescent protein (FP) technology made a great contribution to the development of current biology field. For example, florescent molecular biosensors based on fluorescence resonance energy transfer (FRET), which can be detected between donor and acceptor FPs in proximal, provide powerful tools to visualize dynamic molecular interactions and protein activity in live cells. In addition, physicochemical properties of FPs, for example different pKa, can be applied to design molecular biosensors detecting cellular processes such as pH changes in the autophagic vesicles. In this talk, I will introduce general strategies of FP-based biosensors and present some of recent results in our lab.
Fluorescent protein technology can be further applied to control protein activity by light. In particular, I will introduce an optogenetic strategy to control protein activity by caging the functional domain of the target protein with photodissociable Dronpa dimmer. As the caged domain can be exposed as the dimeric Dronpa became monomers by photoswitching, we can control the protein activity by light. Taken together, fluorescent proteins and advanced imaging technologies enable both optical sensing and control of target protein activity in live cells, which will be powerful tools to understand the real-time molecular events with high spatiotemporal resolutions.