[대학원 생명과학과 세미나 안내]
연사 : 노성훈 교수(서울대학교 생명과학부)
연제 : Visualizing chaperone-directed protein folding pathway
일시 : 2021년 12월 03일 (금) 오후 5시
장소 : 온라인 화상 강의로 진행됩니다.
초청교수 : 우재성 교수
Abstract
Many eukaryotic proteins only fold if assisted by the ring-shaped multisubunit chaperonin TRiC/CCT. To understand how TRiC shapes their folding process, we reconstituted the chaperone-assisted folding pathway. ATP hydrolysis induces TRiC chamber closure and tubulin folding. CryoEM structures at near-atomic resolution reveal subunit-specific interactions with the TRiC chamber wall guide through a set of progressively
folded intermediates to reach the native state. A positively charged TRiC wall patch binds both the negatively charged N-terminal tubulin domain. The disordered CCT C-termini within the chamber promote subsequent folding of the core and middle tubulin domains and formation of the GTP-binding pocket. Since all TRiC/CCT-tubulin contact motifs are conserved, it appears CCT subunits coevolved with obligate substrates to acquire extrinsic information to direct folding of novel proteins with unique topological challenges.